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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
Antimicrobial peptides play a crucial role in insect immunity against invading pathogens. AMPs of biological, especially from eukaryotic cells are explore morein recent days as pathogens are developing resistance against habitual antibiotics. The hemolymph of insect is a dynamic substance, which has many significant functions. Exploring more on these Antimicrobial peptides may serve as a new biotechnological protein based antibiotics. In this present study, hemolymph of immune-challenged moth Eupterote mollifera larvae was isolated and characterized. The Antimicrobial activity of hemolymph was tested against Gram-positive, Gram-negative bacteria and filamentous fungi. The extra cellular protein/peptide was concentrated by AMICON (MWCO-3kDa) ultra-centrifugal filters. The protein concentration was estimated by Bradford dye binding assay and then the haemolytic and haemaggultination activity of the in human RBC cells. Finally, the molecular weights of the protein/peptide were identified by using Tricine SDS-PAGE. Thus the expected antimicrobial protein/peptide, need to be isolated and cloned for large scale antibiotic production.
