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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
2Department of Biotechnology, Sri Krishnadevaraya University, Anantapuramu, Andhra Pradesh, India
3Conservation Scientist and Production Head, Forests by Heartfulness, Kanha Shanthi Vanam-509325, Hyderabad, Telangana, India.
4Department of Botany, Yogi Vemana University, Kadapa-516005, Andhra Pradesh, India
5Phytomedicine Division, Department of Botany, Sri Krishnadevaraya University, Anantapuramu-515003, Andhra Pradesh, India
The fascinating structural alteration of fungi leads to its candidacy as major component for biosynthesis of cellulase enzyme that has major potency is included in several industries including Agriculture, bio-fuel, food, fodder and pharmaceutical sector. In view of its biological significance, newer approaches to maximize its production rate can be through the microbial strain improvement through application of mutagenesis (direct/random). Due to its wide availability, easy genetically alterability and higher ability to produce cellulase; The present study depicts an attempt for strain improvement in one of the fungal species Aspergillus nomius through applications of UV irradiation and induced mutation by chemical mutagen (ethyl methane sulfonate) application for higher yield of cellulase enzyme.From the result average evaluation index for UV treated mutant strains and EMS treated mutants clearly suggested superiority of SKUV7 and EMSSKU5 respectively and the mutant strains like EMSSKU5 and EMSSKU6 showed survival of 5% at 15 minutes of mutagen treatment. From the above study it could be opined that the physical mutagen (UV) treated mutant strain of Aspergillus nomius, SKUV7 and EMS treated mutant strain of Aspergillus nomius, EMSSKU5 are functionally more potent than the control strains of Aspergillus nomiusin terms of production of cellulase enzyme.
