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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
Based on the results of our research group study on gingko using RNA-seq technology, a full-length cDNA sequence of MADS-box gene from Ginkgo biloba was cloned by RT-PCR, which named as GbMADS6 (GenBank accession no. KX061105). The GbMADS6 contains a 735 bp open reading frame(ORF) corresponding to a deduced protein of 245 amino acids, while the estimated isoelectric point (pI) and molecular weight of the putative protein were 5.13 and 59.67 kDa, which had a typical MADS-box domain. Homology analysis indicated that the deduced GbMADS6 protein was highly homologous to other MADS-box Proteins from different species, especially with Lolium perenne MADS4 and Sorghum bicolor MADS1 identities up to 81%. Predicted protein secondary structure showed that GbMADS6 protein contents of the structure with 51.84% of alpha helix (Hh), extended strand (Ee) of 15.10%, random coil (Cc) of 6.94% and beta turn (Tt) of 26.12%. Phylogenetic analysis showed that GbMADS6 has a closer relationship with Pinus tabuliformis MADS protein than with other MADS-box proteins. Those results suggest that GbMADS6 belongs to MADS-box super family and may be involved in regulating the development of the flower.
