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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
The emergence of new infections and increase of multidrug-resistance rises up the urgent need for the development of new antimicrobial agents from natural sources. This study was designed to prepare crude extract from leaves Catha edulis and evaluation for antimicrobial test. The micro-dilution method was applied for the determination of the minimal inhibitory concentration of selected reference pathogens. The antimicrobial activity of crude extracts was potentially inhibit the growth of Gram-negative bacteria comparing with Gram-positive bacteria. Crude leaf extract of Catha edulis showed the highest antibacterial activity against antibiotic resistant Pseudomonas aeruginosa; with a minimum inhibitory concentration value (200 mg/ml) lower than the reference antibiotic tetracycline (250 mg/ml). Higher inhibitory values (300 mg/ml to 500 mg/ml) were also obtained for crude extracts. Phytochemical analysis of crude extract of Catha edulis leaves presented the highest concentrations of amines, ascorbic acid, tannin, and alkaloids. These compounds have important biological activities and could be responsible for at least part of the antibacterial activity of the crude extract of leaves. The Catha edulis crude extract was the most efficient to inhibit the growth of the bacterial strain antibiotics resistant Pseudomonas aeruginosa, and Staphylococcus aureus, which represents an important step for the search and development of a new antimicrobial agent.
