''

Abstract                 Volume:7  Issue-9  Year-2020          Original Research Articles

IJCRBP is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRBP Articles.

Online ISSN : 2349-8080
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorinchiefijcrbp@gmail.com

Antimicrobial, anti-biofilm and anti-quorum sensing compounds from the bark of Ekebergia capensis
Abu F. Meka1, Yadessa M. Ayana2 and Teshome G. Biru1*
1Department of Biology, Natural and Computational Science College, Bule Hora University,P.O. Box 144, Bule Hora, Ethiopia
2Department of Applied Chemistry, School of Applied Natural Science, Adama Science and Technology University,
P.O. Box 1888, Adama, Ethiopia
3Department of Applied Biology, School of Applied Natural Science, Adama Science and Technology University,
P.O. Box 1888, Adama, Ethiopia
*Corresponding author
Abstract:

The main objective of this study was to isolate bioactive compounds from Ekebergia capensis for antimicrobial, anti-biofilm and anti-quorum sensing activity. The bark of E. capensis was extracted using hexane, ethyl acetate and methanol for 72 hours on maceration at room temperature. Antibacterial activity was evaluated against Staphylococcus aureus and antifungal activity against Candida albicans at 5 and 10 mg/mL concentrations. Anti-biofilm activity was tested using test tube and swarming motility test methods and anti-quorum sensing was evaluated using flask incubation test for violacein inhibition production methods. The results showed ethyl acetate extract of E. capensis and its two isolated compounds exhibited antimicrobial, anti-biofilm and anti-quorum sensing activity. Inhibition zones of antimicrobial activities of the extract against S. aureus and C. albicans ranged between 4-20 mm. 10 mg/mL extract showed inhibition of biofilm formation against S. aureus, anti-swarming activities against P. aeruginosa and S. aureus. Inhibition of violacein production against S. aureus, P. aeruginosa, and C. albicans were 72.6%, 67.7% and 69.3% respectively. The ethyl acetate extract, which showed antimicrobials, anti-biofilm, anti-quorum sensing activities, after silica gel column chromatography furnished two compounds namely; diisobutylphtalate and Sandropin B. The chemical structures of these compounds were done using spectroscopic methods including IR and NMR. The isolated compounds also showed strong antimicrobial, anti-biofilm, and anti-quorum sensing activity. This could be useful to manage infectious diseases caused by biofilm forming microorganisms.

Keywords: Antimicrobials Biofilm Ekebergia capensis Extraction Microorganisms Quorum sensing
Download this article as Download

How to cite this article:

Meka, A. F., Ayana, Y. M., Biru, T. G., 2020. Antimicrobial, anti-biofilm and anti-quorum sensing compounds from the bark of Ekebergia capensis.Int.J.Curr.Res.Biosci.Plantbiol. 7(9): 20-28. doi: https://doi.org/10.20546/ijcrbp.2020.709.003
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.