Abstract                 Volume:6  Issue-6  Year-2019          Original Research Articles

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Effect of plant based soya milk extender on Bcl-2 family proteins in cryopreserved Murrah buffalo spermatozoa
R. Mohan1*, H. Batra2 and S. K. Atreja3
1Assistant Professor, Institute of Integrated & Honors Studies, Kurukshetra University, Kurukshetra, Haryana 136119, India
2Senior Research Fellow, ABC Division, National Dairy Research Institute, Karnal, Haryana, India
3Principal Scientist, ABC Division, National Dairy Research Institute, Karnal, Haryana, India
*Corresponding author
Abstract:

The study was designed to investigate the effect of plant based soya milk Tris (SMT)- extender used for cryopreservation of buffalo (Bubalus bubalis) spermatozoa on the expression of Bcl-2 family proteins that regulates the susceptibility of cells to apoptosis. Fresh buffalo semen (control without dilution) was cryopreserved in conventional EYT (20% egg yolk v/v in Tris) and SMT (25% soya milk v/v in Tris) extender and used for the assessment of expression of Bcl-2 family proteins. Proteins extracted from a total number of nine ejaculates from three individual buffalo bulls chosen at random were separated using SDS–PAGE followed by immunoblotting against Bcl-2, Bcl-xl, Bax and Bak. In addition, comet assay was performed to compare sperm DNA integrity in frozen thawed semen samples. The obtained results indicated a significant (p < 0.05) reduction in the expression of Bcl-2, Bax and Bak in cryopreserved semen samples. The results clearly demonstrated a significant (p<0.05) higher expression of Bcl-2 protein in SMT extender as compared to EYT extender indicating its better resistance to cryodamage.

Keywords: Bcl-2 proteins Buffalo spermatozoa Cryopreservation Phytoextender
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How to cite this article:

Mohan, R., Batra, H., Atreja, S. K., 2019. Effect of plant based soya milk extender on Bcl-2 family proteins in cryopreserved Murrah buffalo spermatozoa.Int.J.Curr.Res.Biosci.Plantbiol. 6(6): 34-41. doi: https://doi.org/10.20546/ijcrbp.2019.606.005