|Online ISSN : 2349-8080
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2Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, I.P.N. Cerro Blanco 141, Colinas del Cimatario, Querétaro 76090, Querétaro, México
The presence in oil-contaminated soils of an abundant oil-degrading indigenous microbiota, able to hydrolyze additionally a diversity of bio-polymers by secreting specific enzymes, could explain in part the efficient bioremediation generally observed when oil-polluted soils are composted with a variety of bio-organic wastes. The present study achieved in 12 oil-contaminated soils, demonstrated in all cases their unsuitable nutrient content; however, their oil-degrading bacterial counts fluctuated mainly between 106-107 c.f.u./g soil. The exoenzymatic profile studied in 100 hydrocarbon-degrading bacteria isolated from such soils, gave a percent frequency of positivity of amylases 75, lipases 73, keratinases 67, caseinases 61, elastases 36, chitinases 34, lecithinases 30, chitosanases 25, DNases 19, pectinases 15, cellulases 7 and esterases 6. Most of these isolates (90%) produced 2-5, but some can secrete until 8-10 different exoenzymes.