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2Departement of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University, Makassar, South Sulawesi, 90245, Indonesia
Sugarcane yellow leaf syndrome has been found in South Sulawesi. The aim of this study was to detect phytoplasmas associated with the symptomatic and asymptomatic sugarcane. This study included sampling both symptomatic and asymptomatic leaves, DNA extraction, detection phytoplasma using nested PCR, sequencing and confirmation using Basic Local Alighment Search Tool (BLAST) program. Detection using nested PCR shows the amplification of the 1250 bp DNA band showing the presence of phytoplasma both symptomatic and asymptomatic leaves. This means that sugarcane crop in South Sulawesi has been infected with phytoplasmas.