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Pot experiments were carried out to study the comparative efficiency of individual and combined application of organic manures (vermicompost, cowdung, neem cake) and biofertilizer on growth, biochemical properties and yield of cotton, Gossypium hirsutum L. in botanical garden, Department of Botany, Government Arts College, Musiri. Treatments were organic manures, single, combined application biofertilizer and control (without manures). The obtained results revealed that the response of cotton plant (Gossypium hirsutum L.) showed more significantly increased germination, growth and biochemical characteristics in combined inoculation of different organic manures (biofertilizer + vermicompost + cowdung + neem cake). The minimum germination, growth and biochemical characteristics were observed in the control. On the whole, combined application of organic manures increased the qualitative and quantitative properties of cotton plant significantly when compared to control.