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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
Preparation and Evaluation of Biological Activity of the Sublimated and Cryopreserved Forms of the Herpes Virus of Turkeys
Kazakh Scientific Research Veterinary Institute, Almaty, Republic of Kazakhstan
*Corresponding author
Abstract:
The article contains data on the technology of obtaining and biological activity of the freeze-dried and cryopreserved forms of the turkey herpes virus culture used for the manufacture of the vaccine against Marek's disease. Using the methods of stationary and roller cultivation, a culture biomass of the herpes virus of turkeys was obtained, which was subjected to sublimation and cryopreservation in a mixture of two different stabilizing components. The titration results of these two forms of the vaccine showed that the virus titer was maintained at a level sufficient to be used as a vaccine for the immunization of chickens against Marek's disease.
Keywords: Cell culture Cryopreservation Fibroblasts Sublimation Viral vaccines
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How to cite this article:
Kutumbetov, L.B., Myrzakhmetova, B.Sh., 2017. Preparation and evaluation of biological activity of the sublimated and cryopreserved forms of the herpes virus of turkeysInt.J.Curr.Res.Biosci.Plantbiol. 4(9): 21-23. doi: https://doi.org/10.20546/ijcrbp.2017.409.004
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.