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2Department of Botany and Research Centre, S. T. Hindu College, Nagercoil- 629 002, Tamilnadu, India
3Department of Biotechnology, Malankara Catholic College, Mariagiri, Kaliakkavilai- 629 153, Tamilnadu, India
The effects of plant growth regulators on callus induction were studied in two plants such as Atalantia monophylla and Pamburus missionis. Different concentrations and combinations of hormones were used in MS medium to observe callus induction using leaf as explant. The rate of callus formation varied in different treatments used. The high percentage response of callus induction (90.6 ± 1.15%) was produced on MS medium containing 2,4-D (5 mg/l) from leaf after 17 days in A. monophylla leaf. In case of combination effect of MS media with plant growth hormone (0.25, 0.5, 1 mg/l of IAA and BA), (0.25, 0.5, 1 mg/l of 2,4-D and BA) showed no response in A. monophylla. In P. missionis leaf, high percentage response 93.4 ± 1.50% was produced on MS medium containing 5 mg/ml 2,4-D after 29 days. Among the tested plant growth regulators, MS media supplemented with varying concentration of 2,4-D showed best response for callus induction in A. monophylla and P. missionis leaf explant.