Abstract                 Volume:4  Issue-6  Year-2017          Original Research Articles

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Effect of Sodium Arsenate on Momordica charantia L. Seeds In Vitro
Suman Kumar Ray and Sarmistha Sen Raychaudhuri*
Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92, Acharya Prafulla Chandra Road, Calcutta, 700 009, India
*Corresponding author
Abstract:

Momordica charantia (bitter melon) is a vegetable crop plant of the family Cucurbitaceae. Momordica is a medicinally important plant species and widely used as foodstuff. Arsenic (As) is one of the toxic environmental pollutants, which has recently attracted mass attention because of its chronic and epidemic effects on human health. The absorption of As by land plants is influenced by the concentration of As in the soil. With this perspective the present investigation was undertaken to carry out a comparative analysis on fruit yield and in vitro efficiency of Momordica to establish callus culture from seeds collected from geographically arsenic contaminated and arsenic free two places in West Bengal, India. Calli established in vitro were subjected to Scanning Electron Microscopy (SEM) to determine effect of arsenic on morphological alteration of calli. Seedlings grown in arsenic containing media were used for Atomic Absorption Spectroscopic (AAS) analysis to get a clear picture of the content of eight metals under excess arsenic treatment.

Keywords: Arsenic, Atomic Absorption Spectroscopy, Momordica charantia, Scanning Electron Microscopy
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How to cite this article:

Ray, S. K., Raychaudhuri, S. S., 2017. Effect of sodium arsenate on Momordica charantia L. seeds in vitro.Int.J.Curr.Res.Biosci.Plantbiol. 4(6): 51-61. doi: https://doi.org/10.20546/ijcrbp.2017.406.006