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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
2Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218, USA
3Department of Radiology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
4Hubei Wuchang Experimental Middle School, Wuhan, Hubei 430061, China
5Department of Pathology, Peking University Shougang Hospital, Beijing 100144, China
Vitamin D was discovered about one century ago, which is part of the family of hormones. Vitamin D supplements have been commonly used in medical prescription. Deficiency of Vitamin D3 has been related to various severe diseases, including cardiovascular disease, cancer and diabetes. Therefore, fast and reliable analytic methods for Vitamin D are very important for clinical diagnosis and treatment, as well as for biomedical research. To date, immunoassay, LC-MS/MS and HPLC are three major methods for the qualification and quantitation of Vitamin D. In this paper, we will go through the advantage, disadvantage and limits of each method and provide advice for future analysis of this group of hormone.
HPLC,Immunoassay,LC-MS/MS,Vitamin D,Vitamin D assays
