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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
Mevalonate kinase (MVK) is one of the key rate-limiting enzymes in mevalonic (MVA) pathway of terpenoid biosynthesis. To analyse the function of MVK gene in terpenoid biosynthesis in Chamaemelum nobile, the primers were designed according to the transcript unique sequence of CnMVK from the C. nobile transcriptome dataset. A MVK gene (designated as CnMVK, GenBank accession number KX894317) was cloned from C. nobile using RT-PCR method. The full-length cDNA of CnMVK gene is 1561bp and contains an open reading frame (ORF) of 1206bp, which encoding a 402 amino-acid protein. The theoretical molecular weight and pI of the CnMVK are 42.8kDa and 5.79, respectively. Multi-alignment comparison analysis showed the protein sequence of CnMVK had high similarity with MVK proteins from other plants. Furthermore, CnMVK has similar conserved domains, suggesting CnMVK is one of MVK family mumbers in C. nobile. The present study cloned, analyzed the sequence of CnMVK in C. nobile will provide a foundation for exploring the molecular mechanism of terpenoid biosynthesis in C. nobile.
