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Online ISSN : 2349-8080 Issues : 12 per year Publisher : Excellent Publishers Email : editorinchiefijcrbp@gmail.com |
Phenol and its components are extremely toxic and can easily be isolated from different industrial sewage such as oil refinery, petrochemical industry and mines, especially collier and chemical factories. The presence of these compounds in the environment could cause environmental pollution, especially in water resources. Phenol and its components are majorly toxic to the environment. It is hazardous when exposed to the organism’s surface. Phenol-degrading bacteria exist widely in the environment, and they are usually isolated from phenol contaminated site. The aim of the study was to isolate and identify the phenol degrading bacteria from effluent treatment plant of coir industry. Bacterial strains capable of degrading phenol were isolated from enrichments containing 50 mg/L of phenol. From this 50 ppm sample, the strains were transferred and grown in selective sorbitol media. Five potential strains were selected and were subjected to biochemical characterization and were identified as Brucella sp., Aquaspirillum sp., Erwinia sp., Aeromonas sp. and Moraxella sp. The cultures were subjected to phenol tolerance level of minimum medium containing different concentrations of phenol (200 ppm to 1000 ppm). As the result of the study, it was clear that the selected 5 bacterial strains were able to survive and gave maximum degradation up to 800 mg/L phenol. From the preliminary analysis, the most potent among the selected were Brucella sp. and Aeromonas sp. This study will help to find out the phenol degraders from effluent treatment plant which can be isolated and cultured in large scale and also can be used in waste management.
