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Abstract                 Volume:3  Issue-9  Year-2016          Original Research Articles

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Molecular Cloning and Sequence Analysis of a Novel Geranylgeranyl Diphosphate Synthase Gene (GbGGPPS2) from Ginkgo biloba
Xiaoqiang Li, Weiwei Zhang and Feng Xu*
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
*Corresponding author
Abstract:

Geranylgeranyl diphosphate synthase (GGPPS), a key enzyme in the biosynthesis of ginkgolide, catalyzes the formation of geranylgeranyl diphosphate (GGPP) which is a biosynthetic precursor for ginkgolide. In this study, a novel full-length cDNA encoding GGPPS was isolated, designated as GbGGPPS2 (GenBank accession no. KX756450), from Ginkgo biloba. The full-length cDNA of GbGGPPS2 was 1568 bp and contained a 1149 bp open reading frame (ORF). The molecular weight and isoelectric point of the GbGGPPS2 protein was 129.9 kDa and 5.01, respectively. Multiple alignments analysis revealed that GbGGPPS2 showed extensive homology with GGPPSs from other species and possessed five conservative domains, indicating that the GbGGPPS2 belonged to GGPPS gene family. Phylogenetic tree analysis showed that GbGGPPS2 shared the same ancestor in evolution with other GGPPSs and had a further relationship with other gymnosperm species.

Keywords: GbGGPPS2,Ginkgo biloba,Ginkgolide,Molecular cloning
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How to cite this article:

Li, X., Zhang, W., Xu, F., 2016. Molecular cloning and sequence analysis of a novel geranylgeranyl diphosphate synthase gene (GbGGPPS2) from Ginkgo biloba.Int.J.Curr.Res.Biosci.Plantbiol. 3(9): 40-45. doi: http://dx.doi.org/10.20546/ijcrbp.2016.309.005
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