Abstract                 Volume:3  Issue-7  Year-2016          Original Research Articles

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Impact of Different Carbon Sources, Tri-calcium Phosphate and Auxins on In Vitro Multiplication of Rhizophagus irregularis
B. Mohan Raj1, 2*, R. Bharath Kumar3, G. Venkata Rao1 and K. Sri Rama Murthy1
1R & D Center for Conservation Biology and Plant Biotechnology, Shivashakti Biotechnologies Private Limited, S.R. Nagar, Hyderabad-500 038, Telangana State, India
2Department of Biotechnology, Acharya Nagarjuna University, Guntur-522 510, Andhra Pradesh, India
3Department of Biotechnology, Vignan University, Guntur-522 213, Andhra Pradesh, India
*Corresponding author
Abstract:

In the present study, effect of different combinations and concentrations of carbon source, tri-calcium phosphate and auxins on in vitro multiplication of Rhizophagus irregularis was investigated and rapid production protocol was developed. The high concentration of the sucrose exhibited major role for hyphal growth and spore development in in vitro production of R. irregularis and maltose being the least.  Among all combinations tested, sucrose (10 g/l)+glucose (1 g/l) resulted in well developed mycelial network with profuse spore (59073 IP/100 ml) in M medium in 90 days of incubation along with proportional increase in percentage of colonization and root weight. Tri- calcium phosphate did not significantly enhance the spore development, root colonization and root weight. Different concentrations of IAA and IBA combinations did not affect the root weight and spore development.

Keywords: Arbuscular mycorrhizal fungi. Carbon sources, Rooting hormone, Tri-calcium phosphate,Rhizophagus irregularis
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How to cite this article:

Mohan Raj, B., Bharath Kumar, R., Venkata Rao, G., Sri Rama Murthy, K., 2016. Impact of different carbon sources, tri-calcium phosphate and auxins on in vitro multiplication of Rhizophagus irregularis.

Int.J.Curr.Res.Biosci.Plantbiol. 3(7): 162-166. doi: http://dx.doi.org/10.20546/ijcrbp.2016.307.022