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Abstract                 Volume:3  Issue-7  Year-2016          Original Research Articles

IJCRBP is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCRBP Articles.

Online ISSN : 2349-8080
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Molecular Cloning and Sequence Analysis of GbWRKY31, A Novel Transcription Factor Gene from Ginkgo biloba
Xiaoqiang Li, Jiabao Ye, Xiaohui Wang, Weiwei Zhang and Feng Xu*
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
*Corresponding author
Abstract:

WRKY proteins are a class of plant-specific transcription factors involved in stress response signaling pathways. In this work, a novel WRKY transcription factor gene, named after GbWRKY31, was isolated by RT-PCR method from the Ginkgo biloba. The full-length cDNA of GbWRKY31 was 1738 bp and contained a 1281bp open reading frame (ORF) encoding 427 amino acids. The estimated isoelectric point (pI) and molecular weight of the putative GbWRKY31 protein were 9.10 and 47.05 kDa, respectively. Homology analysis indicated that the deduced GbWRKY31 protein was highly homologous to other WRKY Proteins from different species and had a typical WRKY conserved domain database. Phylogenetic analysis showed that GbWRKY31 belongs to the WRKY IIb group. The results suggest that GbWRKY31 belongs to WRKY families and may be involved in the modulation of senescence and defense in  G. biloba.

Keywords: GbWRKY31,Ginkgo biloba,Modulation,Transcription factor
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How to cite this article:

Li, X., Ye, J., Wang, X., Zhang, W., Xu, F., 2016. Molecular cloning and sequence analysis of GbWRKY31, a novel transcription factor gene from Ginkgo biloba.Int.J.Curr.Res.Biosci.Plantbiol. 3(7): 90-94. doi: http://dx.doi.org/10.20546/ijcrbp.2016.307.013
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